A putative monofunctional glycosyltransferase is expressed in Ralstonia eutropha.

Influence of Matric Potential on Survival and Activity of Genetically Engineered Ralstonia eutropha H850Lr

Although the application of biodegradative genetically engineered micro organisms (GEMs) for bioremediation is very promising, the risks of their release should be assessed before their introduction into the environment. Lux-marked Ralstonia eutropha H850Lr (formerly Alcaligenes eutrophus H850Lr) was introduced into sterile and non-sterile soil microcosms at matric potentials ?2.11, ?30, ?750, ...

The glyoxylate bypass of Ralstonia eutropha.

The glyoxylate bypass genes aceA1 (isocitrate lyase 1, ICL1), aceA2 (isocitrate lyase 2, ICL2) and aceB1 (malate synthase, MS1) of Ralstonia eutropha HF39 were cloned, sequenced and functionally expressed in Escherichia coli. Interposon-mutants of all three genes (DeltaaceA1, DeltaaceA2 and DeltaaceB1) were constructed, and the phenotypes of the respective mutants were investigated. Whereas R. ...

Transcriptional regulation of nitric oxide reduction in Ralstonia eutropha H16.

Nitric oxide reduction in Ralstonia eutropha H16 is catalysed by the quinol-dependent NO reductase NorB. norB and the adjacent norA form an operon that is controlled by the sigma(54)-dependent transcriptional activator NorR in response to NO. A NorR derivative containing MalE in place of the N-terminal domain binds to a 73 bp region upstream of norA that includes three copies of the putative up...

Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture

Formation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by PHA synthase from Ralstonia eutropha.

The acetoacetyl-CoA reductase and the polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha (formerly Alcaligenes eutrophus) were expressed in Escherichia coli, Klebsiella aerogenes, and PHA-negative mutants of R. eutropha and Pseudomonas putida. While expression in E. coli strains resulted in the accumulation of poly(3-hydroxybutyrate) [PHB], strains of R. eutropha, P. putida and K. aero...